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ATCC human malignant glioma cells
Human Malignant Glioma Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 10558 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+malignant+glioma+cells/U-87+MG/bio_rxiv__64898__2026__03__13__711622-59-0-18
Average 99 stars, based on 10558 article reviews

human malignant glioma cells - by Bioz Stars, 2026-09

99/100 stars

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Article Title: Efficient differentiation of human embryonic and induced pluripotent stem cells into functional astrocytes.
Article Snippet: Human high-grade gliomas (hHGG) remain a therapeutic challenge in neuro-oncology despite current multimodality treatments.. We recently demonstrated that murine embryonic stem cell (mESC)-derived astrocytes conditionally expressing proapoptotic genes can successfully be used to induce apoptosis and tumor shrinkage of hHGG tumor in vitro and in an in vivo mouse model.. The first step in the translation of these results to the clinical settings, however, requires availability of human embryonic stem cells (hESC)and/or induced pluripotent cell (hiPSC)-derived astrocytes engineered to express proapoptotic genes.

Article Title: “Footprint-Free” Human Induced Pluripotent Stem Cell-Derived Astrocytes for In Vivo Cell-Based Therapy
Article Snippet: For these experiments, hESC- and hiPSC-derived astrocytes were plated in the top well (5×10 4 cells/well) of 8.0 μm pore size transwell culture dishes (BD Bioscience). .. In the bottom well, culture media or human malignant glioma cells (U87; 5×10 4 cells/well) (American Type Culture Collection) were added and incubated in DMEM+10% serum. ..

Article Title: "Footprint-free" human induced pluripotent stem cell-derived astrocytes for in vivo cell-based therapy.
Article Snippet: For these experiments, hESC- and hiPSC-derived astrocytes were plated in the top well (5 · 104 cells/well) of 8.0 mm pore size transwell culture dishes (BD Bioscience). .. In the bottom well, culture media or human malignant glioma cells (U87; 5 · 104 cells/ well) (American Type Culture Collection) were added and incubated in DMEM + 10% serum. ..

Multiple Displacement Amplification:

Article Title: [ 123 I]Italia: A PARP-Directed Auger Electron-Emitting Agent for Targeted Radionuclide Therapy of Cancer
Article Snippet: .. Human malignant glioma cells (U-87MG), human pancreatic cancer cells (MIA-PaCa2), and breast cancer cells (MDA-MB-231) were purchased from ATCC and maintained in high-glucose Dulbecco’s Modified Eagle’s Medium. .. All media were supplemented with 10% foetal bovine serum (Gibco), 2 mM L-glutamine, 100 units/mL penicillin, and 0.1 mg/mL streptomycin (Gibco).

Modification:

Image Search Results

A MTT assays of U87 and U251 cells with CXCR3 knockdown (shCXCR3) from day 1 to day 4 compared to control (shCtrl). B EdU incorporation assay showed decreased U87 EdU + proliferating cells following CXCR3 knockdown measured by flow cytometry (left). Quantification of signal intensity (right). C . Representative fluorescence images of EdU stained U87 and U251 cells. EdU (red); DAPI (blue). Original magnification ×40 D . Representative images (top) and quantification of colonies (bottom) formed after 14 days of incubation. E Representative images of wound simulation assay after 12 hours of incubation. Original magnification: ×4; Scale bar: 100 mm. F , G Representative images and tumor weight of U87 subcutaneous xenografts from nude mice at day 28 after tumor injection ( n = 6). H , I Representative image and quantification of bioluminescence intensity in U87 orthotopic xenograft expressing shCXCR3 ( n = 5) compared to shCtrl control ( n = 4). The bioluminescence signal was measured twice per week at indicated intervals. Photon flux (p/s/cm 2 /sr) was normalized at day 1 and presented as fold change at day 14, 17, 21, 24, and 28. J . Representative H&E staining of orthotopic implanted U87 cells after mice sacrifice at day 28 at experimental endpoint, Scale bar 800 μm. Error bars indicate mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns (no statistical significance). G , I Error bars indicate mean ± SEM.

Journal: Cell Death Discovery

Article Title: Non-immune targeting of CXCR3 compromises mitochondrial function and suppresses tumor growth in glioblastoma

doi: 10.1038/s41420-025-02449-1

Figure Lengend Snippet: A MTT assays of U87 and U251 cells with CXCR3 knockdown (shCXCR3) from day 1 to day 4 compared to control (shCtrl). B EdU incorporation assay showed decreased U87 EdU + proliferating cells following CXCR3 knockdown measured by flow cytometry (left). Quantification of signal intensity (right). C . Representative fluorescence images of EdU stained U87 and U251 cells. EdU (red); DAPI (blue). Original magnification ×40 D . Representative images (top) and quantification of colonies (bottom) formed after 14 days of incubation. E Representative images of wound simulation assay after 12 hours of incubation. Original magnification: ×4; Scale bar: 100 mm. F , G Representative images and tumor weight of U87 subcutaneous xenografts from nude mice at day 28 after tumor injection ( n = 6). H , I Representative image and quantification of bioluminescence intensity in U87 orthotopic xenograft expressing shCXCR3 ( n = 5) compared to shCtrl control ( n = 4). The bioluminescence signal was measured twice per week at indicated intervals. Photon flux (p/s/cm 2 /sr) was normalized at day 1 and presented as fold change at day 14, 17, 21, 24, and 28. J . Representative H&E staining of orthotopic implanted U87 cells after mice sacrifice at day 28 at experimental endpoint, Scale bar 800 μm. Error bars indicate mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns (no statistical significance). G , I Error bars indicate mean ± SEM.

Article Snippet: Human malignant glioma cell lines U87 and U251 were obtained from the American Type Culture Collection.

Techniques: Knockdown, Control, Flow Cytometry, Fluorescence, Staining, Incubation, Injection, Expressing

A Pathway enrichment analysis using RNA sequencing data from orthotopic xenografts illustrating key signaling pathways involved after CXCR3 ablation. n = 3 per group. B Heatmap analysis highlighting changes in expression of mitochondrial processes in shCtrl and shCXCR3 xenografts. C Representative fluorescence images from orthotopic xenografts showing the subcellular localization of CXCR3 receptor expression in normal brain tissue. Original magnification: ×40. D Representative fluorescence images stained with mitoSOX (red) and Hoechst 33342 (blue). E Flow cytometry analysis of mitochondrial reactive oxygen species production from U87 and U251 cells expressing shCXCR3 compared to control. Red indicates the MitoSOX red stain, which detects superoxide production within the mitochondria of live cells. F Representative fluorescence images in U87 and U251 cells. Green indicates the MitoTracker green dye, which labels mitochondria and provides a measure of total mitochondrial mass within live cells. Red indicates the Mitotracker red CMXROS dye, which accumulates in mitochondria based on their membrane potential. Blue indicates hoechst 33342. Reduced Mitotracker red staining indicates dysfunctional mitochondria with mitochondrial depolarization. G Seahorse mito stress analysis of mitochondrial respiration in U87 cells. (Top) Oxygen Consumption rate (OCR) of U87 cells expressing shCtrl and shCXCR3 under basal conditions, followed by sequential addition of mitochondrial inhibitors (right). Quantification of key respiratory parameters: Non-mit O2 cons non-mitochondrial O2 consumption, Basal basal respiration, Maximal maximal respiration, ATP-linked ATP-linked respiration, Spare Cap. spare capacity. n = 8 per group. Error bars indicate mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns (no statistical significance).

Journal: Cell Death Discovery

Article Title: Non-immune targeting of CXCR3 compromises mitochondrial function and suppresses tumor growth in glioblastoma

doi: 10.1038/s41420-025-02449-1

Figure Lengend Snippet: A Pathway enrichment analysis using RNA sequencing data from orthotopic xenografts illustrating key signaling pathways involved after CXCR3 ablation. n = 3 per group. B Heatmap analysis highlighting changes in expression of mitochondrial processes in shCtrl and shCXCR3 xenografts. C Representative fluorescence images from orthotopic xenografts showing the subcellular localization of CXCR3 receptor expression in normal brain tissue. Original magnification: ×40. D Representative fluorescence images stained with mitoSOX (red) and Hoechst 33342 (blue). E Flow cytometry analysis of mitochondrial reactive oxygen species production from U87 and U251 cells expressing shCXCR3 compared to control. Red indicates the MitoSOX red stain, which detects superoxide production within the mitochondria of live cells. F Representative fluorescence images in U87 and U251 cells. Green indicates the MitoTracker green dye, which labels mitochondria and provides a measure of total mitochondrial mass within live cells. Red indicates the Mitotracker red CMXROS dye, which accumulates in mitochondria based on their membrane potential. Blue indicates hoechst 33342. Reduced Mitotracker red staining indicates dysfunctional mitochondria with mitochondrial depolarization. G Seahorse mito stress analysis of mitochondrial respiration in U87 cells. (Top) Oxygen Consumption rate (OCR) of U87 cells expressing shCtrl and shCXCR3 under basal conditions, followed by sequential addition of mitochondrial inhibitors (right). Quantification of key respiratory parameters: Non-mit O2 cons non-mitochondrial O2 consumption, Basal basal respiration, Maximal maximal respiration, ATP-linked ATP-linked respiration, Spare Cap. spare capacity. n = 8 per group. Error bars indicate mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns (no statistical significance).

Article Snippet: Human malignant glioma cell lines U87 and U251 were obtained from the American Type Culture Collection.

Techniques: RNA Sequencing, Protein-Protein interactions, Expressing, Fluorescence, Staining, Flow Cytometry, Control, Membrane

A The mRNA expression of STAT3 in CXCR3 high and CXCR3 low GBMs from a publicly available database. (TCGA, n = 166). B Western blot analysis of total STAT3 and Phospho-STAT3 (p-STAT3) expression in U87 cells expressing shCtrl and shCXCR3. C Relative cell viability in U87 and U251 cells treated with the CXCR3 inhibitor AMG487 for 72 hours, withglioblastoma stem cell or without the addition of low-dose or high-dose STAT3 activator. The STAT3 activator was used to rescue the effects of CXCR3 inhibition on downstream STAT3 activity. D A hypothetical diagram depicting the proposed mechanism whereby increased CXCR3 activation may lead to enhanced phosphorylation and activation of STAT3 in the mitochondria, which in turn promotes STAT3-mediated redox balance and enhances cancer metabolism as indicated by the increased OCR in GBM. Figure created using BioRender ( https://biorender.com ). (ROS reactive oxygen species, OCR oxygen consumption rate) Error bars indicate mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns (no statistical significance).

Journal: Cell Death Discovery

Article Title: Non-immune targeting of CXCR3 compromises mitochondrial function and suppresses tumor growth in glioblastoma

doi: 10.1038/s41420-025-02449-1

Figure Lengend Snippet: A The mRNA expression of STAT3 in CXCR3 high and CXCR3 low GBMs from a publicly available database. (TCGA, n = 166). B Western blot analysis of total STAT3 and Phospho-STAT3 (p-STAT3) expression in U87 cells expressing shCtrl and shCXCR3. C Relative cell viability in U87 and U251 cells treated with the CXCR3 inhibitor AMG487 for 72 hours, withglioblastoma stem cell or without the addition of low-dose or high-dose STAT3 activator. The STAT3 activator was used to rescue the effects of CXCR3 inhibition on downstream STAT3 activity. D A hypothetical diagram depicting the proposed mechanism whereby increased CXCR3 activation may lead to enhanced phosphorylation and activation of STAT3 in the mitochondria, which in turn promotes STAT3-mediated redox balance and enhances cancer metabolism as indicated by the increased OCR in GBM. Figure created using BioRender ( https://biorender.com ). (ROS reactive oxygen species, OCR oxygen consumption rate) Error bars indicate mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns (no statistical significance).

Article Snippet: Human malignant glioma cell lines U87 and U251 were obtained from the American Type Culture Collection.

Techniques: Expressing, Western Blot, Inhibition, Activity Assay, Activation Assay, Phospho-proteomics

A Structural modeling analysis depicting the binding interactions between the small molecule CXCR3 inhibitor AMG487 and the CXCR3 receptor protein. B Relative cell viability of U87 and U251 cells following 72-hour treatment with increasing concentrations of AMG487 (0, 0.8, 1.6, 3.2 μM) compared to control. Error bars indicate mean ± SD. * p < 0.05, ** p < 0.01 , C , D Representative image and quantification of bioluminescence intensity in orthotopic xenograft in nude mice bearing U87 cells expressing shCXCR3 ( n = 5) compared to control ( n = 4) after AMG487 treatment (10 mg/kg). Bioluminescence intensity was measured twice per week at indicated intervals. Photon flux (p/s/cm 2 /sr) was normalized on day 1 and presented as fold change on days 7, 14, 21, 22, 26, and 29. Error bars indicate mean ± SEM. E Representative brightfield images and quantification of glioblastoma stem cell (GSC) spheroid size (mm 2 ) after 17 days of vehicle (DMSO), low (25 μM) and high (50 μm) dose AMG487 treatment, scale bar 200 μm.

Journal: Cell Death Discovery

Article Title: Non-immune targeting of CXCR3 compromises mitochondrial function and suppresses tumor growth in glioblastoma

doi: 10.1038/s41420-025-02449-1

Figure Lengend Snippet: A Structural modeling analysis depicting the binding interactions between the small molecule CXCR3 inhibitor AMG487 and the CXCR3 receptor protein. B Relative cell viability of U87 and U251 cells following 72-hour treatment with increasing concentrations of AMG487 (0, 0.8, 1.6, 3.2 μM) compared to control. Error bars indicate mean ± SD. * p < 0.05, ** p < 0.01 , C , D Representative image and quantification of bioluminescence intensity in orthotopic xenograft in nude mice bearing U87 cells expressing shCXCR3 ( n = 5) compared to control ( n = 4) after AMG487 treatment (10 mg/kg). Bioluminescence intensity was measured twice per week at indicated intervals. Photon flux (p/s/cm 2 /sr) was normalized on day 1 and presented as fold change on days 7, 14, 21, 22, 26, and 29. Error bars indicate mean ± SEM. E Representative brightfield images and quantification of glioblastoma stem cell (GSC) spheroid size (mm 2 ) after 17 days of vehicle (DMSO), low (25 μM) and high (50 μm) dose AMG487 treatment, scale bar 200 μm.

Article Snippet: Human malignant glioma cell lines U87 and U251 were obtained from the American Type Culture Collection.

Techniques: Binding Assay, Control, Expressing

A , B Representative fluorescence images and flow cytometry analysis of mitochondrial ROS production, MitoSOX red (red) and hoechst 33342 (blue) from U87 and U251 cells treated with AMG487 (dose). C Representative fluorescence images from confocal imaging in cells treated with vehicle (DMSO) and AMG487 (dose) stained with Mitotracker green, Mitotracker red, and hoechst 33342 (blue). D Seahorse analysis of mitochondrial respiration in U87 cells. (left) and OCR of U87 cells treated with vehicle (DMSO) and AMG487 under basal conditions, followed by sequential addition of mitochondrial inhibitors (right). n = 7 per group. E Schematic diagram illustrating the two-photon imaging approach used in the study. Representative two-photon microscopy images of the orthotopic U87 xenograft in mice treated with AMG487 and vehicle (DMSO) for 21 days. Green indicates the rhodamine 123 stain, which labels mitochondrial membrane potential within the tumor cells, hoechst 33342 (blue).

Journal: Cell Death Discovery

Article Title: Non-immune targeting of CXCR3 compromises mitochondrial function and suppresses tumor growth in glioblastoma

doi: 10.1038/s41420-025-02449-1

Figure Lengend Snippet: A , B Representative fluorescence images and flow cytometry analysis of mitochondrial ROS production, MitoSOX red (red) and hoechst 33342 (blue) from U87 and U251 cells treated with AMG487 (dose). C Representative fluorescence images from confocal imaging in cells treated with vehicle (DMSO) and AMG487 (dose) stained with Mitotracker green, Mitotracker red, and hoechst 33342 (blue). D Seahorse analysis of mitochondrial respiration in U87 cells. (left) and OCR of U87 cells treated with vehicle (DMSO) and AMG487 under basal conditions, followed by sequential addition of mitochondrial inhibitors (right). n = 7 per group. E Schematic diagram illustrating the two-photon imaging approach used in the study. Representative two-photon microscopy images of the orthotopic U87 xenograft in mice treated with AMG487 and vehicle (DMSO) for 21 days. Green indicates the rhodamine 123 stain, which labels mitochondrial membrane potential within the tumor cells, hoechst 33342 (blue).

Article Snippet: Human malignant glioma cell lines U87 and U251 were obtained from the American Type Culture Collection.

Techniques: Fluorescence, Flow Cytometry, Imaging, Staining, Microscopy, Membrane